【實驗記錄】2010.09.24

《Coating Cantilever》

7:50

1. wash cantilevers by cold PBS three times
2. add avadin and put them in R.P. for 30 min
3. wash cantilevers by cold PBS twice
4. add con-A and put them in R.P. for 1 hour (due to analomy class)
5. wash cantilevers by cold PBS twice and add ddWater

《Collagen Coating》

10:00

1. Take dishes coated collagen out of the fridge
2. Wash by cold PBS twice
3. Add PBS and seal with parafilm

《Cell Subculture》

16:00

切完後細胞計數: 84 (1mL trypsin + 4mL DMEM)

1. 取1.5 mL加入10 cm dish
2. 取500μL離心，抽掉上清液加1500μL PBS，AFM用
3. (下次可以再少一點)