【實驗記錄】2010.09.07

《Gel 2-2》

Time: 09:30

1. 照一般UV15分鐘
2. 倒掉collagen，washed by cold PBS
3. washed by DMEM three times
4. add DMEM and seal dishes with parafilm, store in the fridge.

《Coating Cantilever 2-2》

從ecubator中拿出來發現昨天忘記封parafilm了，所以biotin幾乎都蒸發掉了。

(不知道這樣會不會造成影響?)

1. Washed by iced PBS for 4 times (fullfill the whole dish twice)
2. Add avadin and put them in room temperature for 30 min.
3. Washed by iced PBS for 3 times (fulfill the whole dish once)
4. Add con-A and put them in room temperature for 30 min
5. Washed by iced PBS for 3 times (fulfill the whole dish once)

   (0號針在過程中斷掉了)

6. Add ddWater and seal the dish with parafilm, store in the fridge.

p.s. 四號針在乃哥整理冰箱的過程中不小心被打破了dish，所幸針沒有斷，所以再加滿dd Water，封parafilm冰在冰箱

《Cell》

今天下午看細胞時，發現細胞死掉了(細胞懸浮在medium中，都沒有貼附在dish上)

跟學長討論過後，再解凍細胞(液態氮存取編號4-1)

Time:17:30

P39→P40

 《Coating Cantilever 3-1》

由於四號針又多了一個變數，所以乾脆再co一支新的針，五號針

18:00 加入Biotin-BSA, 封parafilm → ecubator