《Coating Canteliver 3-2》
7:45
- washed by cold PBS three times (fulfill the whole dish)
- Add avadin and put it in room temperature
- washed by cold PBS three times (fulfill the whole dish)
- Add con-A and put it in room temperature. (At this step, the twin adhesive is not sticky anymore, so I change the twin adhesive again.)
- Add dd water and seal with parafilm, store in the fridge.
《Cell Culture》
Change medium.
《Gel》
Add DMEM and refridge in the fridge.
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